Benson's Microbiological Applications : Laboratory Manual in General Microbiology, Short Version,9780072823974

Benson's Microbiological Applications : Laboratory Manual in General Microbiology, Short Version

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Edition: 9th
ISBN13: 9780072823974
ISBN10: 0072823976
Format: Paperback
Pub. Date: 2004-03-31
Publisher(s): McGraw-Hill Science/Engineering/Math
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Summary

The classic resource for undergraduate microbiology laboratory courses just keeps getting better. The self-contained, clearly illustrated exercises and four-color format make Microbiological Applications: A Laboratory Manual in General Microbiology the ideal lab manual. Appropriate for either a majors or non-majors lab course, Benson assumes no prior organic chemistry course has been taken.

Table of Contents

Preface vii
Basic Microbiology Laboratory Safety ix
Biosafety Levels for Selected Infectious Agents xii
Microorganisms Used in These Lab Exercises and Their BSL Ratings xiii
PART 1 Microscopy
1(28)
Brightfield Microscopy
3(8)
Darkfield Microscopy
11(4)
Phase-Contrast Microscopy
15(8)
Microscopic Measurements
23(6)
PART 2 Survey of Microorganisms
29(28)
Protozoans, Algae, and Cyanobacteria
31(14)
Ubiquity of Bacteria
45(4)
The Fungi: Yeasts and Molds
49(8)
PART 3 Manipulation of Microorganisms
57(24)
Aseptic Technique
59(10)
Pure Culture Techniques
69(12)
PART 4 Staining and Observation of Microorganisms
81(32)
Smear Preparation
83(4)
Simple Staining
87(2)
Negative Staining
89(2)
Capsular Staining
91(4)
Gram Staining
95(4)
Spore Staining: Two Methods
99(4)
Acid-Fast Staining: Ziehl-Neelsen Method
103(4)
Motility Determination
107(6)
PART 5 Culture Methods
113(38)
Culture Media Preparation
115(10)
Cultivation of Anaerobes
125(6)
Enumeration of Bacteria: The Standard Plate Count
131(10)
Slime Mold Culture
141(6)
Slide Culture: Molds
147(4)
PART 6 Bacterial Viruses
151(20)
Determination of a Bacteriophage Titer
155(6)
Isolation of Phage from Flies
161(6)
Phage Typing
167(4)
PART 7 Environmental Influences and Control of Microbial Growth
171(46)
Temperature: Effects on Growth
173(4)
Temperature: Lethal Effects
177(4)
pH and Microbial Growth
181(4)
Water Activity and Osmotic Pressure
185(4)
Ultraviolet Light: Lethal Effects
189(4)
Oligodynamic Action
193(4)
Evaluation of Alcohol: Its Effectiveness As an Antiseptic
197(4)
Antimicrobic Sensitivity Testing: The Kirby-Bauer Method
201(6)
Evaluation of Antiseptics: The Filter Paper Disk Method
207(4)
Effectiveness of Hand Scrubbing
211(6)
PART 8 Identification of Unknown Bacteria
217(38)
Morphological Study of Unknown Bacterium
219(6)
Cultural Characteristics
225(4)
Physiological Characteristics: Oxidation And Fermentation Tests
229(8)
Physiological Characteristics: Hydrolytic Reactions
237(4)
Physiological Characteristics: Biochemical Tests
241(8)
Use of Bergey's Manual
249(6)
PART 9 Miniaturized Multitest Systems
255(30)
Enterobacteriaceae Identification: The API 20E System
257(6)
Enterobacteriaceae Identification: The Enterotube II System
263(8)
O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System
271(8)
Staphylococcus Identification: The API Staph-Ident System
279(6)
PART 10 Applied Microbiology
285(28)
Bacterial Counts of Foods
287(4)
Bacteriological Examination of Water: Qualitative Tests
291(6)
The Membrane Filter Method
297(4)
Reductase Test
301(4)
Microbial Spoilage of Canned Food
305(4)
Microbiology of Alcohol Fermentation
309(4)
PART 11 Medical Microbiology and Immunology
313(60)
The Staphylococci: Isolation and Identification
315(8)
The Streptococci: Isolation and Identification
323(12)
Gram-Negative Intestinal Pathogens
335(6)
Slide Agglutination Test: Serological Typing
341(2)
Slide Agglutination (Latex) Test: For S. aureus Identification
343(4)
Tube Agglutination Test: The Heterophile Antibody Test
347(4)
White Blood Cell Study: The Differential WBC Count
351(6)
Blood Grouping
357(6)
A Synthetic Epidemic
363(10)
Appendix A Tables 373(8)
Appendix B Indicators, Stains, Reagents 381(4)
Appendix C Media 385(4)
Appendix D Identification Charts 389(10)
Appendix E The Streptococci: Classification, Habitat, Pathology, and Biochemical Characteristics 399(4)
Reading References 403(2)
Index 405

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