Formalin-Fixed Paraffin-Embedded Tissues

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Edition: 1st
Format: Hardcover
Pub. Date: 2011-02-01
Publisher(s): Humana Pr Inc
List Price: $219.99

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Summary

Presenting an area of research that intersects with and integrates diverse disciplines, including genomics, epigenetics, proteomics, and cellular biology, among others, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Compiled in order to provide researchers with up-to-date methodological information pertaining to the utilization of genomic, transcriptomic, and proteomic data in diagnosis, prognosis, and tailored therapy, the ultimate aim of this volume is to decipher diseases at a molecular level. Divided into multiple convenient chapters, this detailed book covers various techniques to construct and utilize tissue arrays, it also provides detailed protocols in immunohistochemistry, immunofluorescence, fluorescent and chromogenic in situ hybridization, and ultimately introduces protocols for FFPET microdissection and nucleic acids extraction for their utilization in advanced techniques such as microarray CGH, DNA methylation and pyrosequencing. The volume also discusses FFPET research from an ethical standpoint and concludes with a chapter on novel tissue fixative. Written in the highly successful Methods in Molecular Biology'„¢ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this vital field of research.

Table of Contents

Prefacep. vii
Contributorsp. xi
Regulatory and Ethical Issues on the Utilization of FFPE Tissues in Researchp. 1
Tissue Microarrays: Construction and Usesp. 23
Standardization in Immunohistologyp. 37
Multiple Immunofluorescence Labeling of Formalin-Fixed Paraffin-Embedded Tissuep. 69
Microwaves for Chromogenic In Situ Hybridizationp. 79
Automated Analysis of FISH-Stained HER2/neu Samples with Metaferp. 91
Laser Capture Microdissection of FFPE Tissue Sections Bridging the Gap Between Microscopy and Molecular Analysisp. 105
Nucleic Acids Extraction from Laser Microdissected FFPE Tissue Sectionsp. 117
Microarray-Based CGH and Copy Number Analysis of FFPE Samplesp. 131
Microarray Profiling of DNA Extracted from FFPE Tissues Using SNP 6.0 Affymetrix Platformp. 147
Whole Genome Amplification of DNA Extracted from FFPE Tissuesp. 161
Pyrosequencing of DNA Extracted from Formalin-Fixed Paraffin-Embedded Tissuep. 181
Analysis of DNA Methylation in FFPE Tissues Using the MethyLight Technologyp. 191
RT-PCR Gene Expression Profiling of RNA from Paraffin-Embedded Tissues Prepared Using a Range of Different Fixatives and Conditionsp. 205
RT-PCR-Based Gene Expression Profiling for Cancer Biomarker Discovery from Fixed, Paraffin-Embedded Tissuesp. 239
MicroRNA Isolation from Formalin-Fixed, Paraffin-Embedded Tissuesp. 259
Gene Expression Profiling of RNA Extracted from FFPE Tissues: NuGEN Technologies' Whole-Transcriptome Amplification Systemp. 269
Protein Mass Spectrometry Applications on FFPE Tissue Sectionsp. 281
An Alternative Fixative to Formalin Fixation for Molecular Applications: The RCL2®-CS100 Approachp. 297
Indexp. 309
Table of Contents provided by Ingram. All Rights Reserved.

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