A Laboratory Guide to the Mammalian Embryo,9780195142266

A Laboratory Guide to the Mammalian Embryo

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ISBN13: 9780195142266
ISBN10: 0195142268
Format: Paperback
Pub. Date: 2004-01-08
Publisher(s): Oxford University Press
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Summary

This book pulls together the full range of cell culture, biochemical, microscopic, and genetic techniques to study the early mammalian embryo. Until now, there has never been such a comprehensive compendium, though there have been more focused books of protocol, such as Manipulating the MouseEmbryo, from Cold Spring Harbor. This book is intended to appeal to all constituencies, from basic experimental science to clinical and animal science applications.

Table of Contents

Contributors ix
Abbreviations xiii
1. Follicle Development in Vitro 3(21)
Norah Spears, Evelyn E. Telfer, and Alison A. Murray
2. Preparation of Gametes, in Vitro Maturation, in Vitro Fertilization, and Embryo Recovery and Transfer 24(17)
Michelle Lane and David K. Gardner
3. Culture of the Mammalian Preimplantation Embryo 41(21)
David K. Gardner and Michelle Lane
4. Assessment of Preimplantation Embryo Development and Viability 62(14)
Denny Sakkas, Michelle Lane, and David K. Gardner
5. Micromanipulation of Gametes 76(23)
Gianpiero D. Palermo, Queenie V. Neri, Takumi Takeuchi, Lucinda L. Veeck, and Zev Rosenwaks
6. Micromanipulation: Biopsy 99(26)
Joyce C. Harper and Alpesh Doshi
7. Analysis of Intracellular Ions in Embryos: pH and Calcium 125(14)
Michelle Lane, Jay M. Baltz, and David K. Gardner
8. Assessment of Nutrient Uptake, Metabolite Production, and Enzyme Activity 139(15)
David K. Gardner and Michelle Lane
9. Metabolic Pathway Activity 154(11)
Don Rieger
10. Confocal Imaging of Structural Molecules in Mammalian Gametes 165(19)
Calvin Simerly, Ricardo Moreno, João Ramalho-Santos, Laura Hewitson, and Gerald Schatten
11. Fluorescent in situ Hybridisation for Detection of Aneuploidy in Single Human Blastomeres for Preimplantation Genetic Diagnosis 184(9)
Leeanda Wilton
12. Assessment of Ploidy, Telomere Length, and Telomerase Activity in Oocytes and Embryos 193(24)
W. Allan King, Dean H. Betts, Wafa Bureau, and Parvathi K. Basrur
13. Microscale RNA Isolation from Mammalian Embryos 217(8)
Christine Wrenzycki, Andrew J. Watson, and Michele Calder
14. Relative mRNA Transcript Abundance in Early Embryos by Reverse Transcription-Polymerase Chain Reaction 225(12)
Paul A. De Sousa, and Andrew J. Watson
15. Characterization of Novel Genes during Early Development by Application of Differential Display RT-PCR 237(10)
David R. Natale and Andrew J. Watson
16. Two-Dimensional Protein Gel Database Analysis of Embryos, Oocytes, and Oocyte-Associated Granulosa Cells 247(11)
Keith E. Latham, Gula Nourjanova, Karen Wigglesworth, and John J. Eppig
17. Antisense Disruption of Gene Expression in the Preimplantation Embryo 258(12)
Lisa C. Barcroft, D. Holstead Jones-Taggart, and Andrew J. Watson
18. Gene Subtraction and Analysis 270(9)
Marc-André Sirard and Claude Robert
19. Analysis of Apoptosis in the Preimplantation Embryo 279(19)
Daniel R. Brison, Anthony D. Metcalfe, Debra J. Bloor, Helen R. Hunter, Gerard Brady, and Susan J. Kimber
20. Cytoplasmic Signaling and Cell Cycle Control in the Mouse Egg and Embryo 298(15)
D. Page Baluch, Christine M. Pauken, and David G. Capco
21. Human Embryonic Stem Cells 313(21)
Susan E. Lazendorf, Catherine A. Boyd, and Diane L. Wright
22. Spermatogonial Transplantation 334(18)
Makoto C. Nagano
23. Somatic Cell Nuclear Transfer 352(23)
Paul De Sousa, William Ritchie, Andras Dinnyes, Tim King, Lesley Paterson, and Ian Wilmut
24. Cryobiology: Slow Freezing Vitrification of Embryos 375(16)
Tetsunori Mukaida and Magosaburo Kasai
Index 391

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