Phage Display of Peptides and Proteins : A Laboratory Manual
by Kay, Brian K.; Winter, Jill; McCafferty, JohnFormat: Paperback
Pub. Date: 1996-08-01
Publisher(s): Academic Pr
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Summary
Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols. Phage Display of Peptides and Proteins: A Laboratory Manual reviews the literature and outlines the strategies for maximizing the successful application of phage display technology to one's research. It contains the most up-to-date protocols for preparing peptide affinity reagents, monclonal antibodies, and evolved proteins. Key Features * Prepared by experts in the field * Provides proven laboratory protocols, troubleshooting, and tips * Includes maps, sequences, and sample data * Contains extensive and up-to-date references
Table of Contents
| Foreword | |
| Biology of the Filamentous Bacteriophage | |
| Principles and Applications of Phage Display | |
| Vectros for Phage Display | |
| Construction of Random Peptide Libraries in Bacteriophage | |
| Construction and Screening of Antibody Display Libraries | |
| Phagemeid-Displayed Peptide Libraries | |
| Phage Libraries Displaying Random Peptides | |
| Derived Form a Target Sequence | |
| Display and Selection of Proteins on Genetic Packages | |
| A Cdna Cloning System Based on Filamentous Phage: Selection and Enrichment of Functional Gene Products By Protein/Ligand | |
| Interactions Made Possible By Linkage of Recogntion and Replication Functions | |
| Multicombinational Libraries and Combinational Infection: Practical Considerations for Vector Design | |
| Use of Filamentous Virus Particles as Immunogens | |
| Screening Phage Displayed Random Peptide Libraries | |
| Phage Display Factors Affecting Planning Efficieny | |
| Preparation of Second Generation Phage Libraries | |
| Nonradioactive Sequencing of Random Peptide Recombinant Phage | |
| Measurement of Peptide Binding Affinities Using Fluorescence Polarization | |
| Table of Contents provided by Publisher. All Rights Reserved. |
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