Platelet Proteomics Principles, Analysis, and Applications

The purpose of the book is to introduce platelets, and their functional role in thrombotic and cardiovascular disease, justifying the relevance of platelet proteomics research. Focus then shifts to the recent developments on mass spectrometry (MS)-based proteomics.  This chapter shows potential applications for platelet proteomics not yet carried out.  It includes examples of post-translational modifications (PTMs) analysis in platelets.

The second part of the book focuses on the main research done so far on platelet proteomics. This includes general proteome mapping by non-gel based separation methods (MudPit), analysis of the general platelet proteome and signaling cascades by gel-based separation methods (2-DE), sub-proteome analyses (secretome/releasate, membrane proteins, organelles). Finally, the last section links the platelet transcriptome and application to disease.  This section is highly relevant and includes chapters on proteomics, transcriptomics, functional genomics, systems biology, and their applications to platelet-related diseases.

Dr. Ángel García is currently a Ramón y Cajal Research Fellow at the Pharmacology Department, Universidade de Santiago de Compostela, Spain His main research is the area of platelet proteomics, with special emphasis in cardiovascular research and platelet signaling studies. He has written over twenty publications in peer-reviewed journals and has been an invited lecturer at the Platelets 2010 International Symposium (Jerusalem, Israel), and the XXII Congress of the International Society on Thrombosis and Haemostasis, held in Boston, United States, in 2009, where he chaired a session on novel approaches to understanding platelet biology. Sr. Garcia was a postdoctoral research associate of the Proteomics Group at the Oxford Glycobiology Institute, University of Oxford ((UK), from 2001 to 2005.

Dr. Yotis Senis  is currently a British Heart Foundation Research Fellow at the University of Birmingham, United Kingdom. He is a senior member of the Birmingham Platelet Group. Dr. Senis’ research interests include the identification of novel platelet surface glycoproteins and determining their biological and molecular functions. He is also interested in the functional roles of protein tyrosine phosphatases in platelets and thrombosis. Dr. Senis has been an invited lecturer at the 2010 International Symposium (Jerusalem, Israel) and the Gordon Research Conference on the Cell Biology of Megakaryocytes and Platelets (Galveston, Texas). He has written over twenty publications on platelet signaling and thrombosis.

Contributors.

Foreword.

Preface.

Acronyms.

PART I GENERAL OVERVIEW: PLATELETS, SAMPLE PREPARATION, AND MASS SPECTROMETRY-BASED PROTEOMICS.

1 Platelets and Their Role in Thrombotic and Cardiovascular Disease: The Impact of Proteomic Analysis (Ronald G. Stanley, Katherine L. Tucker, Natasha E. Barrett, and Jonathan M. Gibbins).

2 Mass-Spectrometry-Based Proteomics: General Overview and Posttranslational Modification Analysis in the Context of Platelet Research (Julia Maria Burkhart and Albert Sickmann).

3 Sample Preparation Variables in Platelet Proteomics for Biomarker Research (Maria Zellner and Rudolf Oehler).

PART II ANALYSIS OF THE PLATELET PROTEOME: GLOBAL APPROACHES AND SUBPROTEOMES.

4 Two-Dimensional Gel Electrophoresis: Basic Principles and Application to Platelet Signaling Studies (Angel Garcia).

5 The Platelet Membrane Proteome (Yotis A. Senis).

6 Proteomics of Platelet Granules, Organelles, and Releasate (James P. McRedmond).

7 The Platelet Microparticle Proteome (David M. Smalley).

8 N-Terminal Combined Fractional Diagonal Chromatographic (COFRADIC) Analysis of the Human Platelet Proteome (Francis Impens, Kenny Helsens, Niklaas Colaert, Lennart Martens, Joel Vandekerckhove, and Kris Gevaert).

PART III INTEGRATED "OMICS" AND APPLICATION TO DISEASE.

9 Serial Analysis of Gene Expression (SAGE) For Studying the Platelet and Megakaryocyte Transcriptome (Michael G. Tomlinson).

10 The Application of Microarray Analysis and Its Integration with Proteomics for Study of Platelet-Associated Disorders (Dmitri V. Gnatenko and Wadie F. Bahou).

11 Platelet Functional Genomics (Isabelle I. Salles, Marie N. O’Connor, Daphne C. Thijssen-Timmer, Katleen Broos, and Hans Deckmyn).

12 Systems Biology to Study Platelet-Related Bleeding Disorders (Jan-Willem N. Akkerman and Bernard De Bono).

13 Platelet Proteomics in Transfusion Medicine (Thomas Thiele, Leif Steil, Uwe V¨olker, and Andreas Greinacher).

14 Cardiovascular Proteomics (Fernando Vivanco, Fernando de la Cuesta, Maria G. Barderas, Irene Zubiri, and Gloria A´lvarez-Llamas).

Index.