
Bioseparations Science and Engineering
by Harrison, Roger G.; Todd, Paul W.; Rudge, Scott R.; Petrides, DemetriRent Textbook
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Summary
Author Biography
Table of Contents
Preface | |
All chapters (except Chapter 12) end with the following sections: Summary, Nomenclature, Problems, and References | |
Preface | |
Introduction to Bioproducts and Bioseparations | |
Instructional Objectives | |
Broad Classification of Bioproducts | |
Small Biomolecules | |
Primary Metabolites | |
Secondary Metabolites | |
Summary of Small Biomolecules | |
Macromolecules: Proteins | |
Primary Structure | |
Secondary Structure | |
Tertiary Structure | |
Example | |
Effect of a Reducing Agent on Protein Structure and Mobility | |
Quaternary Structure | |
Prosthetic Groups and Hybrid Molecules | |
Functions and Commercial Uses of Proteins | |
Stability of Proteins | |
Recombinant Protein Expression | |
Macromolecules: Nucleic Acids and Oligonucleotides | |
Macromolecules: Polysaccharides | |
Particulate Products | |
Introduction to Bioseparations: Engineering Analysis | |
Stages of Downstream Processing | |
Example | |
:Initial Selection of Purification Steps | |
Basic Principles of Engineering Analysis | |
Process and Product Quality | |
Criteria for Process Development | |
The Route to Market | |
The Chemical and Applications Range of the Bioproduct | |
Documentation of Pharmaceutical Bioproducts | |
GLP and cGMP | |
Formulation | |
Analytical Methods | |
Instructional Objectives | |
Specifications | |
Assay Attributes | |
Precision | |
Accuracy | |
Specificity | |
Linearity, Limit of Detection, and Limit of Quantitation | |
Range | |
Robustness | |
Analysis of Biological Activity | |
Animal Model Assays | |
Cell-Line-Derived Bioassays | |
In Vitro Biochemical Assays | |
Example | |
:Coupled Enzyme Assay for Alcohol Oxidase | |
Analysis of Purity | |
Electrophoretic Analysis | |
Example | |
:Estimation of the Maximum Temperature in an Electrophoresis Gel | |
High Performance Liquid Chromatography (HPLC) | |
Mass Spectrometry | |
Coupling of HPLC with Mass Spectrometry | |
UV Absorbance | |
Example | |
:Determination of Molar Absorptivity | |
CHNO/Amino Acid Analysis (AAA) | |
Example | |
:Calculations Based on CHNO Analysis | |
Protein Assays | |
Enzyme-Linked Immunosorbent Assay | |
Gas Chromatography | |
DNA Hybridization | |
ICP/MS (AA) | |
Dry Weight | |
Microbiology Assays | |
Sterility | |
Bioburden | |
Endotoxin | |
Virus and Phage | |
Cell Lysis and Flocculation | |
Instructional Objectives | |
Some Elements of Cell Structure | |
Prokaryotic Cells | |
Eukaryotic Cells | |
Cell Lysis | |
Osmotic and Chemical Cell Lysis | |
Mechanical Methods of Lysis | |
Flocculation | |
The Electric Double Layer | |
Example | |
:Dependence of the Debye Radius on the Type of Electrolyte | |
Forces between Particles and Flocculation by Electrolytes | |
Example | |
:Sensitivity of Critical Flocculation Concentration to Temperature and Counter-Ion Charge Number | |
The Schulze-Hardy Rule | |
Table of Contents provided by Publisher. All Rights Reserved. |
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